Last updated: June 2026 · Reviewed June 2026 · Built by the InjectBuddy team
How many mL is 100mg testosterone per week? volumes and units at every vial strength
A 100mg-per-week testosterone dose works out to 0.5 ml (50 units) on a 200mg/ml vial and 0.4 ml (40 units) on a 250mg/ml vial using a U-100 insulin syringe. The dose in milligrams never changes; the milliliters you draw shift with the strength printed on the vial, because a more concentrated oil packs more testosterone into each milliliter. This guide works through the conversion formula, shows the full draw chart at every common vial strength, covers once-weekly and twice-weekly splits, and answers the questions people ask most.
Key takeaways
- Volume (ml) = dose (mg) ÷ concentration (mg/ml). 100mg is 1.0 ml at 100mg/ml, 0.5 ml at 200mg/ml, 0.4 ml at 250mg/ml.
- On a U-100 syringe, multiply milliliters by 100 to read units: 0.5 ml = 50 units, 0.4 ml = 40 units.
- Splitting 100mg into two 50mg shots halves the per-injection draw but keeps the weekly total at 100mg.
- Always confirm the strength on the vial label first — 200 vs 250mg/ml changes every number by 20%.
Confirm any figure for your exact strength in the free Testosterone (TRT) dose calculator.
100mg testosterone per week is how many ml?
The milliliters you draw depend entirely on the strength printed on the vial. The dose in milligrams stays the same; the volume changes because a stronger vial packs more milligrams into each milliliter. The formula never changes:
Volume (ml) = dose (mg) ÷ concentration (mg/ml)
Testosterone cypionate and enanthate are most commonly supplied at 200mg/ml; older 100mg/ml presentations still exist (the DEPO-Testosterone label lists both), and some compounded products run at 250mg/ml. For a once-weekly 100mg dose:
- At 100mg/ml: 100 ÷ 100 = 1.0 ml
- At 200mg/ml: 100 ÷ 200 = 0.5 ml
- At 250mg/ml: 100 ÷ 250 = 0.4 ml
So the same 100mg prescription is a full milliliter at 100mg/ml, half a milliliter at 200mg/ml and four-tenths at 250mg/ml. If you are unsure why the volume moves, the relationship is unpacked in dose vs volume explained and how mg/ml works.
Dose 100mg, vial 200mg/ml. 100 ÷ 200 = 0.5 ml. Multiply back to check: 0.5 ml × 200mg/ml = 100mg. The arithmetic closes, so 0.5 ml is correct.
Converting ml to syringe units
Many people inject testosterone with a U-100 insulin syringe because the fine markings make a small oil volume easier to read. The "U-100" means 100 units per milliliter — the same convention insulin labels use — so 1 ml = 100 units and 0.1 ml = 10 units. To convert, multiply milliliters by 100.
0.5 ml × 100 = 50 units. 0.4 ml × 100 = 40 units. So 100mg/week is 50 units from a 200mg/ml vial, or 40 units from a 250mg/ml vial.
The unit marks measure volume on the barrel, not the amount of drug. The same 50-unit mark holds a different number of milligrams depending on vial strength — covered in U-100 syringe units explained.
Once-weekly: the full draw chart
This table sets out the complete once-weekly draw for a fixed 100mg dose at the three strengths you are most likely to meet. The U-100 unit column is simply the milliliter figure times 100.
| Vial strength | Dose | Volume (ml) | U-100 units |
|---|---|---|---|
| 100 mg/ml | 100 mg | 1.00 ml | 100 units |
| 200 mg/ml | 100 mg | 0.50 ml | 50 units |
| 250 mg/ml | 100 mg | 0.40 ml | 40 units |
Read the strength off the label, not the box, and confirm it before every draw. A common error is assuming 200mg/ml when the vial is actually 250mg/ml.
You draw 50 units (0.5 ml) believing the vial is 200mg/ml, but it is really 250mg/ml. 0.5 ml × 250mg/ml = 125mg — 25mg more than intended. On a 250mg/ml vial, 100mg is 40 units, not 50.
A legacy 100mg/ml DEPO-style vial: 100 ÷ 100 = 1.0 ml = the full 100-unit barrel of a 1 ml U-100 syringe. The same 100mg dose that is half a syringe at 200mg/ml fills the whole syringe here.
How this is calculated (the methodology)
There are only two arithmetic steps, and both are reversible so you can always check yourself:
- Milligrams to milliliters: divide the dose by the concentration. 100mg ÷ 200mg/ml = 0.5 ml. The mg unit cancels, leaving ml.
- Milliliters to units: multiply by 100, because a U-100 syringe is defined as 100 units per ml. 0.5 ml × 100 = 50 units.
To verify, run it backwards: units ÷ 100 = ml, then ml × concentration = mg. If that does not return your prescribed milligrams, the strength you entered is wrong. The method is generalised in how to calculate injection volume.
You have 40 units on the barrel and want to confirm the dose on a 250mg/ml vial. 40 ÷ 100 = 0.4 ml. 0.4 ml × 250mg/ml = 100mg. Confirmed.
Splitting 100mg into two injections a week
Some protocols split the weekly total into two smaller injections, for example every 3.5 days. Splitting does not change the weekly milligram total; it halves the amount per injection. For 100mg/week split in two, each injection is 50mg.
Run the same formula on the per-injection dose of 50mg:
- At 200mg/ml: 50 ÷ 200 = 0.25 ml = 25 units
- At 250mg/ml: 50 ÷ 250 = 0.20 ml = 20 units
100mg/week on a 200mg/ml vial, twice weekly: 0.25 ml (25 units) on Monday and 0.25 ml (25 units) on Thursday. 2 × 0.25 ml = 0.5 ml, matching the once-weekly 0.5 ml total exactly.
Whether to split is a clinical decision, not a maths one — testosterone cypionate peaks roughly two days after an injection, which is part of why some protocols favour more frequent, smaller doses. The mechanics are in what is a split dose and the timing trade-offs in daily vs twice weekly vs every 3.5 days.
Once-weekly vs twice-weekly side by side
The table below shows both schedules so the per-injection draw is easy to compare. Note the weekly total is identical in every row — only the size of each individual draw changes.
| Schedule | Strength | Per injection | Units | Weekly total |
|---|---|---|---|---|
| 1×/week | 200 mg/ml | 0.50 ml | 50 units | 100 mg |
| 2×/week | 200 mg/ml | 0.25 ml | 25 units | 100 mg |
| 1×/week | 250 mg/ml | 0.40 ml | 40 units | 100 mg |
| 2×/week | 250 mg/ml | 0.20 ml | 20 units | 100 mg |
50mg on a 250mg/ml vial is only 0.20 ml (20 units). At that small a volume the syringe dead space and meniscus matter, so a 0.3 ml or 0.5 ml U-100 syringe reads more precisely than a 1 ml barrel.
A smaller per-injection volume is one reason some people find twice-weekly injections more comfortable, but comfort and absorption are individual. To push further to every-other-day doses, see splitting 100mg weekly into EOD microdoses.
Avoiding the common errors
- Confirm vial strength first. 200mg/ml and 250mg/ml look similar on a label but change the draw by 20 percent. A dedicated chart for those two strengths is in the 200 vs 250mg/ml draw chart.
- Match the syringe to the scale. The unit conversions here assume a U-100 syringe. A syringe marked only in milliliters has no "units" — read the ml figure instead.
- Testosterone undecanoate vs cypionate dosing
- Testosterone esters explained
- Pick a barrel you can read. A 0.5 ml insulin syringe shows a 0.4–0.5 ml draw more clearly than a 1 ml barrel.
To confirm the figures for your exact strength, enter your dose and concentration in the Testosterone (TRT) dose calculator. This is a measurement tool, not medical advice; the 100mg/week figure is a typical starting dose cited in clinical references, but dosing decisions belong with a prescriber.
So, how many mL is 100mg testosterone per week?
At the most common vial strengths, 100mg per week is 0.5 ml (50 units) from a 200mg/ml vial and 0.4 ml (40 units) from a 250mg/ml vial. The formula is always the same: volume (ml) = dose (mg) divided by concentration (mg/ml), then multiply by 100 to read U-100 units. Always confirm the strength on your vial label before drawing, then enter your exact numbers into the Testosterone (TRT) dose calculator to get an instant result.
FAQs
How many mL is 100mg testosterone per week?
How many units is 100mg of testosterone on an insulin syringe?
If I split 100mg per week into two injections, how much is each?
Does splitting the dose change how much I draw in total?
Why does the same 100mg dose need a different volume on different vials?
Sources
- DailyMed (U.S. National Library of Medicine). DEPO-Testosterone (testosterone cypionate injection, solution) prescribing information — 100 mg/mL and 200 mg/mL strengths. dailymed.nlm.nih.gov.
- Bhasin S, Brito JP, Cunningham GR, et al. Testosterone Therapy in Men With Hypogonadism: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2018;103(5):1715–1744. pubmed.ncbi.nlm.nih.gov/29562364.
- Sizar O, Schwartz J. Male Hypogonadism. StatPearls. NCBI Bookshelf; updated 2024 (100 mg weekly starting dose; peak levels ~2 days post-injection). ncbi.nlm.nih.gov/books/NBK532933.
- DailyMed (U.S. National Library of Medicine). Humulin R (insulin human) injection, 100 units per mL (U-100) — confirms the U-100 = 100 units/mL convention. dailymed.nlm.nih.gov.
- DailyMed (U.S. National Library of Medicine). DEPO-Testosterone dosage and administration (replacement dosing for hypogonadism). dailymed.nlm.nih.gov.
This guide is for general educational purposes only and does not constitute medical advice. Always follow your prescriber's specific instructions.