Peptide Calculators
Last updated: June 2026
BPC-157 + TB-500 Blend 250/250 mcg: Units to Draw
This page works through one of the most-asked blend questions: how many syringe units a 250 mcg BPC-157 plus 250 mcg TB-500 dose comes to after reconstitution. It is a measurement worksheet, not a dosing recommendation.
What "bpc-157 tb-500 blend 250mcg units" actually asks
The phrase covers a specific, common setup: a combined dose of 250 mcg BPC-157 and 250 mcg TB-500 drawn into a single insulin syringe, and the question of how many units that is on a U-100 barrel. The answer is never a fixed number. It depends entirely on how concentrated your reconstituted peptide is, which depends on the vial size in milligrams and how much bacteriostatic water you added.
There are two physical arrangements people mean by "blend":
- Two separate vials — a BPC-157 vial and a TB-500 vial, each reconstituted on its own, drawn one after the other into the same syringe.
- One dual-peptide vial — a single vial already containing both peptides as a lyophilised powder, reconstituted once, drawn once.
The math below covers both. If you would rather enter your own numbers, the BPC-157 + TB-500 blend calculator outputs each draw and the combined total for you. Note that neither BPC-157 nor TB-500 has established human dosing from controlled trials, so the figures here are arithmetic conversions only, not a protocol — see the safety note at the end.
The core formula (units = mL x 100)
Every result on this page comes from two steps. First, the concentration of the reconstituted vial:
Concentration (mcg/mL) = vial content (mcg) ÷ bacteriostatic water added (mL)
Then the draw volume for your dose, converted to U-100 insulin units:
Draw volume (mL) = dose (mcg) ÷ concentration (mcg/mL)
Units = draw volume (mL) × 100
On a U-100 syringe, 1 mL = 100 units, so 0.10 mL = 10 units and 0.05 mL = 5 units. If you are new to reading the barrel, how to read an insulin syringe and U-100 syringe units explained cover the markings. The unit conversion itself is the same regardless of which peptide you are drawing.
250/250 mcg from two separate vials
This is the most common case: a 5 mg BPC-157 vial and a 5 mg TB-500 vial, each reconstituted with the same amount of water, drawn in sequence into one syringe. Because the two doses are equal (250 mcg each) and the vials are matched (same mg, same water), the two draws are identical.
Example
Both vials are 5 mg (5,000 mcg), each reconstituted with 2 mL of bacteriostatic water.
Concentration of each vial: 5,000 mcg ÷ 2 mL = 2,500 mcg/mL.
BPC-157 draw: 250 mcg ÷ 2,500 mcg/mL = 0.10 mL = 10 units.
TB-500 draw: 250 mcg ÷ 2,500 mcg/mL = 0.10 mL = 10 units.
Draw the BPC-157 first, then the TB-500 into the same barrel. Combined total: 0.20 mL = 20 units.
Because you fill to 10 units, then continue to 20 units, the plunger stops at the 20-unit mark when both draws are in. That is well within a 0.3 mL (30-unit) syringe.
Concentration table: units per 250 mcg draw
This table shows the units for a single 250 mcg draw at common reconstitution volumes. For a 250/250 blend from two matched vials, double the unit figure to get the combined total in the syringe.
| Vial size | BAC water | Concentration | 250 mcg draw | Both peptides (250/250) |
|---|---|---|---|---|
| 5 mg | 1 mL | 5,000 mcg/mL | 0.05 mL = 5 units | 0.10 mL = 10 units |
| 5 mg | 2 mL | 2,500 mcg/mL | 0.10 mL = 10 units | 0.20 mL = 20 units |
| 5 mg | 3 mL | 1,667 mcg/mL | 0.15 mL = 15 units | 0.30 mL = 30 units |
| 10 mg | 2 mL | 5,000 mcg/mL | 0.05 mL = 5 units | 0.10 mL = 10 units |
| 10 mg | 3 mL | 3,333 mcg/mL | 0.075 mL = 7.5 units | 0.15 mL = 15 units |
| 10 mg | 5 mL | 2,000 mcg/mL | 0.125 mL = 12.5 units | 0.25 mL = 25 units |
The last column only applies when both vials are reconstituted to the same concentration. If your BPC-157 and TB-500 vials differ in size or water volume, calculate each draw separately and add them — see the next section.
When the two vials are NOT matched
If the vials differ, the two halves of the 250/250 dose draw to different unit marks, and you must add them. The combined total is what determines whether the dose fits your syringe.
Example
BPC-157: 5 mg vial + 2 mL water = 2,500 mcg/mL. TB-500: 10 mg vial + 5 mL water = 2,000 mcg/mL.
BPC-157 draw: 250 mcg ÷ 2,500 = 0.10 mL = 10 units.
TB-500 draw: 250 mcg ÷ 2,000 = 0.125 mL = 12.5 units.
Combined: 10 + 12.5 = 22.5 units (0.225 mL).
Drawing to 22.5 units on a 0.3 mL syringe is awkward to read precisely. If your combined draw lands on an ugly fraction, the fix is usually to reconstitute the two vials to the same concentration so both halves draw to round marks. The idea of dialling reconstitution to land on clean units is covered in how much BAC water should I add?
250/250 mcg from one dual-peptide vial
Some suppliers sell a single vial already containing both peptides, for example 5 mg BPC-157 + 5 mg TB-500 as a combined 10 mg of powder. Here you reconstitute once and draw once, because both peptides are in solution together at a fixed ratio.
Example
Dual vial: 5 mg BPC-157 + 5 mg TB-500 (10 mg total powder), reconstituted with 2 mL of bacteriostatic water.
Total concentration: 10,000 mcg ÷ 2 mL = 5,000 mcg/mL combined — which is 2,500 mcg/mL of each peptide because they are in a 1:1 ratio.
To get 250 mcg of each, you need 500 mcg of total peptide: 500 mcg ÷ 5,000 mcg/mL = 0.10 mL = 10 units, drawn in one pull.
The key difference: with a 1:1 dual vial you cannot dose the two peptides independently — every draw delivers them in the same fixed ratio. If the vial is a different ratio (say 2:1), the single draw still works, but the mcg of each peptide per unit changes, so read the label and recompute. This single-vial path is the one the angle of this page maps to most directly. Drawing both peptides together is also the standard approach described in the peptide blend calculator guide.
Checking your draw fits the syringe
A 250/250 blend almost always fits a 0.3 mL (30-unit) insulin syringe at typical concentrations, since the combined draws in the table above range from 10 to 30 units. Two practical checks before you draw:
- Combined total under your barrel capacity. 30 units is the ceiling for a 0.3 mL syringe; if the sum of both draws exceeds it, step up to a 0.5 mL (50-unit) barrel or split into two injections.
- Both halves land on readable marks. Half-unit draws (like 7.5 or 12.5) are possible but harder to measure accurately; matched concentrations avoid them.
For more on choosing barrel size and why the smallest syringe that fits your volume is easiest to read, see 0.3 mL vs 0.5 mL vs 1 mL syringes. Run your own vial sizes and doses through the blend calculator to confirm the units before drawing.
FAQs
How many units is a 250/250 mcg BPC-157 + TB-500 blend?
It depends on concentration. With two matched 5 mg vials each reconstituted with 2 mL of bacteriostatic water (2,500 mcg/mL each), each 250 mcg draw is 0.10 mL = 10 units, for a combined 0.20 mL = 20 units. At 1 mL of water per 5 mg vial (5,000 mcg/mL), each draw is 5 units and the combined total is 10 units. Always compute from your own vial size and water volume.
Do I draw BPC-157 and TB-500 separately or together?
If you have two separate reconstituted vials, draw each in sequence into the same syringe (BPC-157 first to its unit mark, then TB-500 up to the combined mark). If you have one dual-peptide vial containing both, you reconstitute once and draw both together in a single pull, because they are already in solution at a fixed ratio.
What if my combined draw is an awkward number like 22.5 units?
That happens when the two vials are reconstituted to different concentrations, so each 250 mcg half draws to a different mark. The cleanest fix is to reconstitute both vials to the same concentration so each half lands on a round unit. Matching a 5 mg vial to 2 mL of water on both gives 10 units each, 20 units total.
Is a 250/250 mcg dose safe?
BPC-157 and TB-500 are not approved medicines and have no established human dosing from controlled clinical trials. This page only converts a given mcg figure into syringe units; it is not a recommendation that 250/250 mcg is safe or effective. Using research peptides without medical supervision carries real, poorly characterised risks. Speak to a qualified clinician before using anything injectable.
Does the blend calculator handle a single dual-peptide vial?
The calculator is built for the two-vial approach, where each peptide is reconstituted separately and drawn into one syringe. For a single dual-peptide vial in a 1:1 ratio, treat the total powder as one concentration: total mcg divided by water gives the combined mcg/mL, then divide your total peptide dose (250 + 250 = 500 mcg) by that to get the single draw volume.
Read next
Bpc 157 Tb500 Blend GuideSources
- Duzel A, Vlainic J, Antunovic M, et al. Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights. World J Gastroenterol. 2017;23(48):8465-8488. PMID: 29358856. pmc.ncbi.nlm.nih.gov/articles/PMC5752708.
- Goldstein AL, Hannappel E, Kleinman HK. Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421-429. PMID: 16099219. pubmed.ncbi.nlm.nih.gov/16099219.
- U.S. Food and Drug Administration. Certain Bulk Drug Substances for Use in Compounding that May Present Significant Safety Risks (Category 2 substances nominated under sections 503A/503B). www.fda.gov/drugs/human-drug-compounding/certain-bulk-drug-substances-use-compounding-may-present-significant-safety-risks.
- U.S. Pharmacopeia. General Chapter <797> Pharmaceutical Compounding - Sterile Preparations: standards for preparing injectable sterile medications. www.usp.org/compounding/general-chapter-797.
- DailyMed (U.S. National Library of Medicine). HUMULIN R - insulin human injection, solution: FDA-approved label stating 100 units per mL (U-100). dailymed.nlm.nih.gov/dailymed/drugInfo.cfm?setid=9ec3e28a-cea9-4e45-9057-e5bf8e37014c.
This guide is for general educational purposes only and does not constitute medical advice. Always follow your prescriber's specific instructions.