Last updated: June 2026 · Reviewed June 2026 · Built by the InjectBuddy team
How do you dose a BPC-157 + TB-500 blend? mg to units guide
To dose a BPC-157 + TB-500 blend vial, add bacteriostatic water to the single vial, then divide the total peptide mass (both peptides added together) by that water volume to get the blend's mg/mL concentration; your dose in mg divided by that concentration, multiplied by 100, gives the units to draw on a U-100 insulin syringe. This guide explains what a blend vial is, works through the three-step formula with a dose chart and seven worked examples, and answers the questions people ask most.
Key takeaways: A blend vial fixes the BPC-157:TB-500 ratio for you, so the maths collapses to a single concentration. Total blend mg ÷ BAC water mL = mg/mL. Dose ÷ mg/mL × 100 = units. Both peptides are preclinical — this is a maths reference, not a dosing recommendation.
Open Blend Calculator →What a blend vial is
Many research suppliers sell BPC-157 and TB-500 pre-combined in one lyophilised vial at a fixed ratio — commonly labelled by total milligrams and ratio, such as a 10 mg blend at 5 mg BPC-157 / 5 mg TB-500, or an 11 mg blend at 5 mg / 6 mg. Because both peptides share the same vial and the same water, you reconstitute and draw once, not twice. That is the whole appeal: one calculation, one injection.
The trade-off is that the ratio is locked at the factory. With separate vials you can dial any BPC-157:TB-500 split you like; with a blend you take whatever ratio the label states. So the first thing to read off the vial is the total mg — that is the number your concentration maths uses.
Honesty note: BPC-157 (a synthetic gastric pentadecapeptide) and TB-500 (a synthetic Thymosin β4 fragment) are both investigational research peptides. The supporting evidence is overwhelmingly animal and in-vitro — there is no established human dose, no FDA approval, and no clinical standard for a combined blend. Everything below is concentration arithmetic, not medical advice.
How this is calculated
A blend vial behaves like any single peptide vial, except the "peptide content" is the sum of both peptides:
1. Blend concentration (mg/mL) = Total blend mg ÷ BAC water added (mL)
2. Draw volume (mL) = Your dose (mg) ÷ Blend concentration (mg/mL)
3. Units on a U-100 syringe = Draw volume (mL) × 100
The subtlety: when you draw a "1 mg blend dose" from a 5 mg / 5 mg vial, you are taking 0.5 mg BPC-157 and 0.5 mg TB-500 in that single draw — the ratio rides along automatically. If the label is 5 mg / 6 mg (11 mg total), the same 1 mg draw delivers roughly 0.45 mg BPC-157 and 0.55 mg TB-500. The syringe units only ever track the total concentration.
Blend mg → mg/mL → units
This chart reconstitutes whole blend vials with 2 mL of bacteriostatic water and shows the units for a 1 mg total-blend dose. Halve the dose, halve the units.
| Blend vial (total mg) | BAC water | Concentration | 1 mg dose draw | Units (U-100) |
|---|---|---|---|---|
| 5 mg (2.5/2.5) | 2 mL | 2.5 mg/mL | 0.40 mL | 40 units |
| 10 mg (5/5) | 2 mL | 5 mg/mL | 0.20 mL | 20 units |
| 11 mg (5/6) | 2 mL | 5.5 mg/mL | 0.18 mL | 18 units |
| 15 mg (7.5/7.5) | 2 mL | 7.5 mg/mL | 0.13 mL | 13 units |
| 20 mg (10/10) | 2 mL | 10 mg/mL | 0.10 mL | 10 units |
Worked examples
10 mg ÷ 2 mL = 5 mg/mL. Draw = 1 ÷ 5 = 0.20 mL.
Concentration still 5 mg/mL. Draw = 0.5 ÷ 5 = 0.10 mL.
20 mg ÷ 3 mL = 6.67 mg/mL. Draw = 1 ÷ 6.67 = 0.15 mL.
11 mg ÷ 2 mL = 5.5 mg/mL. Draw = 1 ÷ 5.5 = 0.182 mL.
That 1 mg draw is ~0.45 mg BPC-157 + ~0.55 mg TB-500 (the 5:6 ratio).
5 mg ÷ 1 mL = 5 mg/mL. Draw = 0.5 ÷ 5 = 0.10 mL.
15 mg ÷ 2 mL = 7.5 mg/mL. Draw = 1.5 ÷ 7.5 = 0.20 mL.
10 mg ÷ 5 mL = 2 mg/mL. Draw = 2 ÷ 2 = 1.00 mL — exactly fills a 1 mL insulin syringe.
Reconstituting the blend vial
- Read the total mg and ratio off the label (e.g. "10 mg · 5/5"). The total mg is what your maths uses.
- Wipe the septum with an alcohol swab; let it dry 10–15 seconds.
- Draw your chosen BAC water volume and inject it slowly down the inside wall of the vial. Swirl gently — never shake — until fully dissolved.
- The solution should be clear and colorless. Discard if cloudy or if particles persist.
- Label the vial with the concentration and reconstitution date, then refrigerate.
Less water means a higher mg/mL and fewer units per dose; more water means a lower mg/mL and more units. Choosing the water volume so a typical dose lands between 10 and 40 units keeps the draw easy to read.
Enter your blend's total mg, BAC water, and dose to get the exact draw in mL and units instantly.
Open the Blend Calculator →So, how do you dose a BPC-157 + TB-500 blend vial?
You divide the total blend mg by the volume of bacteriostatic water you add to get mg/mL, then divide your dose by that concentration and multiply by 100 to read the units on a U-100 insulin syringe. For a common 10 mg (5/5) vial reconstituted with 2 mL, a 1 mg dose is 5 mg/mL concentration, 0.20 mL, and 20 units. Use the BPC-157 + TB-500 blend calculator to enter your exact vial, water volume, and dose and get the draw instantly.
Frequently asked questions
How do you dose a BPC-157 + TB-500 blend vial?
Do I add the two peptides together when calculating concentration?
How is a blend vial different from using two separate vials?
What ratio is in a typical BPC-157 + TB-500 blend?
What if a dose makes the draw exceed 1 mL?
Sources
BPC-157 and TB-500 are investigational peptides; the evidence below is predominantly preclinical (animal and in-vitro). Neither is FDA-approved, and no human dose for a combined blend is established. The arithmetic here is standard concentration maths, not a clinical protocol.
- Gwyer D, Wragg NM, Wilson SL. Gastric pentadecapeptide body protection compound BPC 157 and its role in accelerating musculoskeletal soft tissue healing. Cell Tissue Res. 2019;377(2):153–159. — Review noting efficacy "yet to be confirmed in humans."
- Chang CH, Tsai WC, Lin MS, Hsu YH, Pang JH. The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration. J Appl Physiol. 2011;110(3):774–780.
- Sikiric P, Seiwerth S, Rucman R, et al. Stable gastric pentadecapeptide BPC 157: novel therapy in gastrointestinal tract. Curr Pharm Des. 2011;17(16):1612–1632.
- Goldstein AL, Hannappel E, Sosne G, Kleinman HK. Thymosin β4: a multi-functional regenerative peptide. Basic properties and clinical applications. Expert Opin Biol Ther. 2012;12(1):37–51.
- Goldstein AL, Hannappel E, Kleinman HK. Thymosin β4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421–429.
This guide is a mathematical reference only and does not constitute medical advice. BPC-157 and TB-500 are not approved for human use; consult a qualified clinician before using any injectable.