Peptide Blend Guide
Last updated: May 2026
BPC-157 + TB-500 Blend Guide
This guide covers how to reconstitute BPC-157 and TB-500 from separate vials, calculate the draw volume for each, and combine both into a single injection. It is a mathematical and procedural reference — not medical advice.
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The two-vial approach
BPC-157 and TB-500 are sold as separate lyophilised vials. Each must be reconstituted independently with its own bacteriostatic water before use. Rather than injecting each peptide separately, many users draw from both vials into the same syringe — reducing total injections to one.
The key arithmetic challenge is that each vial has its own concentration, determined by how much BAC water was added to it. You must calculate each peptide's draw volume separately, then combine them.
What is TB-500?
TB-500 is a synthetic version of Thymosin Beta-4, a naturally occurring peptide involved in actin regulation and cellular processes. It is sold as a lyophilised research peptide in 5 mg or 10 mg vials, identical in format to BPC-157 vials. Like BPC-157, TB-500 must be reconstituted with bacteriostatic water before injection, and doses are measured in mcg.
TB-500 doses tend to be larger than BPC-157 doses in absolute mcg terms. A common example is 250–500 mcg BPC-157 combined with 500–2,000 mcg TB-500 per injection, though individual protocols vary widely.
Reconstituting both vials
Reconstitute each vial separately with its own chosen BAC water volume:
- Wipe both vial septums with separate alcohol swabs. Allow to dry 10–15 seconds.
- Draw your chosen BAC water volume for BPC-157 into a syringe. Inject slowly down the inside wall of the BPC-157 vial. Swirl gently until dissolved. Do not shake.
- Repeat with a fresh syringe for TB-500, using the BAC water volume appropriate for that vial.
- Both solutions should be clear and colourless. Discard if cloudy or particulate.
You can use different BAC water volumes for each vial — the calculator handles each independently.
Calculating draw volumes
For each peptide independently:
Formula (applies to each vial separately)
Concentration = Vial content (mcg) ÷ BAC water added (mL)
Draw (mL) = Dose (mcg) ÷ Concentration (mcg/mL)
Units (U-100) = Draw (mL) × 100
Example — 250 mcg BPC-157 + 500 mcg TB-500, 5 mg vials, 2 mL BAC water each
BPC-157: 5,000 mcg ÷ 2 mL = 2,500 mcg/mL → 250 ÷ 2,500 = 0.10 mL
TB-500: 5,000 mcg ÷ 2 mL = 2,500 mcg/mL → 500 ÷ 2,500 = 0.20 mL
BPC-157: 10 units · TB-500: 20 units
Total in syringe: 0.30 mL = 30 units
Calculate draw volumes for both peptides and the combined total for any vial size, BAC water, and dose combination.
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Draw order and technique
- Using a fresh insulin syringe, draw the calculated BPC-157 volume from the BPC-157 vial.
- Without changing the syringe, insert the needle into the TB-500 vial and draw the TB-500 volume. Both are now in the syringe.
- Verify the total volume against your expected combined units on the syringe scale.
- Clean the injection site. Inject subcutaneously — common sites: abdomen, upper thigh, upper arm.
- Dispose of the needle safely without recapping.
If the combined volume exceeds 1 mL, consider using a 1.5 mL or 2 mL tuberculin syringe, reconstituting with less BAC water to increase concentration, or splitting into two separate injections at different sites.
Concentration reference
Both BPC-157 and TB-500 use the same concentration formula. This table applies to either vial:
| BAC water | 5 mg vial (5,000 mcg) | 10 mg vial (10,000 mcg) |
|---|
| 1 mL | 5,000 mcg/mL | 10,000 mcg/mL |
| 2 mL | 2,500 mcg/mL | 5,000 mcg/mL |
| 3 mL | 1,667 mcg/mL | 3,333 mcg/mL |
| 5 mL | 1,000 mcg/mL | 2,000 mcg/mL |
Frequently asked questions
Why combine BPC-157 and TB-500 in the same injection?
BPC-157 and TB-500 are both research peptides used individually and in combination. They have different primary structures and receptor affinities, and combining them into one injection reduces the total number of injections per session. Because they are sold as separate lyophilised vials, each is reconstituted independently with its own BAC water. Drawing both into the same syringe before injection is the standard method for a combined dose. The blend calculator handles the separate concentration calculations for each vial and outputs individual draw volumes plus the combined total.
What ratio of BPC-157 to TB-500 is typically used?
There is no universally standardised ratio for BPC-157 and TB-500 in a blend — reported use varies widely. BPC-157 is typically dosed at 200–500 mcg per injection, while TB-500 is often dosed at 500–2,000 mcg per injection. Common ratios include 1:2 (e.g., 250 mcg BPC-157 with 500 mcg TB-500) and 1:4 (250 mcg with 1,000 mcg). Pre-mixed blend vials from some research suppliers fix a specific ratio. When using separate vials, you can choose any ratio you need. The blend calculator accepts independent dose inputs for each peptide so any combination can be calculated.
What happens if the combined draw exceeds 1 mL?
Standard insulin syringes hold up to 1 mL (100 units on U-100 scale). If the combined BPC-157 and TB-500 draw exceeds 1 mL, you have three options: reconstitute each vial with less BAC water to increase concentration and reduce draw volumes; use a larger syringe (e.g., 2 mL or 3 mL tuberculin syringe); or split the protocol into two separate injections, drawing BPC-157 and TB-500 separately at different sites. The blend calculator flags a warning when the combined draw exceeds 1 mL so you can plan accordingly.
What is TB-500 and how does it differ from BPC-157?
TB-500 is a synthetic version of Thymosin Beta-4, a naturally occurring peptide involved in actin regulation and cellular processes. Like BPC-157, TB-500 is sold as a lyophilised research peptide in 5 mg or 10 mg vials that must be reconstituted before use. The reconstitution method and draw volume calculation are identical for both peptides. TB-500 doses tend to be larger in absolute mcg than BPC-157 doses, so it often requires a larger fraction of the syringe when combined. The blend calculator handles each vial's concentration independently, since different BAC water volumes may be used for each.
Sources
Both BPC-157 and TB-500 (Thymosin Beta-4 fragment) are research peptides with primarily preclinical study data. Neither is FDA-approved for human use. The reconstitution and injection preparation steps in this guide are based on standard peptide handling practices applicable to both compounds. The blend calculation method (treating each vial's concentration independently) is standard arithmetic — there is no evidence base specific to the combined blend.
Sikiric P, et al. Stable gastric pentadecapeptide BPC 157: novel therapy in gastrointestinal tract. Curr Pharm Des. 2011;17(16):1612–1632. ·
Goldstein AL, Hannappel E, Kleinman HK. Thymosin β4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421–429.
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