Last updated: · Maintained by Sam Calder
How do you dose a BPC-157 + TB-500 blend? mg to units guide
To dose a BPC-157 + TB-500 blend vial, add bacteriostatic water to the single vial, then divide the total peptide mass (both peptides added together) by that water volume to get the blend's mg/mL concentration; your dose in mg divided by that concentration, multiplied by 100, gives the units to draw on a U-100 insulin syringe. This guide explains what a blend vial is, works through the three-step formula with a dose chart and seven worked examples, and answers the questions people ask most.
Key takeaways: A blend vial fixes the BPC-157:TB-500 ratio for you, so the maths collapses to a single concentration. Total blend mg ÷ BAC water mL = mg/mL. Dose ÷ mg/mL × 100 = units. Both peptides are preclinical — this is a maths reference, not a dosing recommendation.
Open Blend Calculator →What a blend vial is
Many research suppliers sell BPC-157 and TB-500 pre-combined in one lyophilized vial at a fixed ratio — commonly labeled by total milligrams and ratio, such as a 10 mg blend at 5 mg BPC-157 / 5 mg TB-500, or an 11 mg blend at 5 mg / 6 mg. Because both peptides share the same vial and the same water, you reconstitute and draw once, not twice. That is the whole appeal: one calculation, one injection.
The trade-off is that the ratio is locked at the factory. With separate vials you can dial any BPC-157:TB-500 split you like; with a blend you take whatever ratio the label states. So the first thing to read off the vial is the total mg — that is the number your concentration maths uses.
A blend vial or two separate vials?
The word “blend” can describe two different setups. A dual-peptide vial contains both BPC-157 and TB-500 in the same container, at a fixed ratio. Add water once, calculate from the total powder mass, and make one draw. Two separate vials contain one peptide each. Each vial has its own concentration, so calculate each draw independently and combine the volumes in the syringe. The two setups are not interchangeable: a single-vial calculation does not describe two vials, and two separate-vial calculations do not describe a fixed-ratio blend.
Separate vials let you choose the amount of each peptide independently. A dual vial ties the amounts together according to its label ratio. For example, if the label lists equal amounts, each draw contains equal amounts; with an unequal ratio, a single draw cannot change that proportion. Read the amount of each peptide on the label rather than assuming that every product called a blend is 1:1.
For two vials, use the concentration printed or calculated for each vial, then apply the desired dose for that peptide. Add the two resulting volumes to find the total syringe fill. If both vials have the same concentration and the doses are equal, both draws use the same number of units. If their concentrations differ, the unit marks differ too. The blend calculator models two independent vial inputs, so use it for separate vials; use the total mass and fixed ratio when reasoning about a dual vial.
250/250 mcg from two separate vials
For a 250 mcg amount of each peptide, a common separate-vial example uses one 5 mg BPC-157 vial and one 5 mg TB-500 vial. If each is mixed with the same amount of water, each draw is the same size. The table shows the draw from each vial and the combined amount in the syringe. The concentration and draw are calculated separately for BPC-157 and TB-500.
| Each vial | Water in each vial | Concentration per vial | Each 250 mcg draw | Combined 250/250 draw |
|---|---|---|---|---|
| 5 mg | 1 mL | 5,000 mcg/mL | 0.05 mL = 5 units | 0.10 mL = 10 units |
| 5 mg | 2 mL | 2,500 mcg/mL | 0.10 mL = 10 units | 0.20 mL = 20 units |
| 5 mg | 3 mL | about 1,667 mcg/mL | 0.15 mL = 15 units | 0.30 mL = 30 units |
| 10 mg | 2 mL | 5,000 mcg/mL | 0.05 mL = 5 units | 0.10 mL = 10 units |
For each row, the concentration is vial content divided by water volume. The draw in mL is 250 mcg divided by that concentration, and U-100 units are mL multiplied by 100. For example, a 5 mg vial contains 5,000 mcg; with 2 mL of water it is 2,500 mcg/mL. Each 250 mcg draw is 0.10 mL, or 10 units, so two matched vials add to 0.20 mL, or 20 units. Enter 5 mg, 2 mL, and 250 mcg for each component in the calculator to check the same two draws.
When separate vials have different concentrations
Do not double one draw when the vials are mismatched. Work out each concentration and each volume on its own, then add the volumes. For example, a 5 mg BPC-157 vial mixed with 2 mL contains 2,500 mcg/mL; 250 mcg corresponds to 0.10 mL, or 10 units. A 10 mg TB-500 vial mixed with 5 mL contains 2,000 mcg/mL; 250 mcg corresponds to 0.125 mL, or 12.5 units. Exact arithmetic gives 0.225 mL, or 22.5 unit-equivalents. The calculator rounds each vial's unit display to a whole number, so it shows 10 + 13 = 23 units and a total volume of 0.23 mL. Enter each vial strength and water amount with a 250 mcg dose to check the same inputs.
The combined volume determines whether the draw fits your syringe, while each individual draw determines where to stop before switching vials. A combined total that fits the barrel may still include a fractional unit mark. Check the barrel capacity and graduations before drawing; do not round away a difference between the two concentrations.
250/250 mcg from one dual-peptide vial
A single dual-peptide vial is reconstituted once and drawn once. Its ratio is fixed by the amount of each peptide in the powder. The concentration calculation uses the total mass in the vial, while the ratio tells you how that total is divided between BPC-157 and TB-500. A 1:1 label has equal peptide amounts in each draw; a different ratio gives different amounts. You cannot independently adjust one peptide while keeping the other unchanged in a single draw. Check the product label and use a calculator that models the actual vial type before interpreting a result.
The 250/250 label describes equal amounts of the two peptides, not one universal syringe mark. With two separate vials, calculate and add two draws. With a dual vial, whether a single draw contains equal amounts depends on its labeled ratio. This distinction is why the examples above use separate-vial inputs only; they should not be applied to a dual vial.
Honesty note: BPC-157 (a synthetic gastric pentadecapeptide) and TB-500 (a synthetic Thymosin β4 fragment) are both investigational research peptides. The supporting evidence is overwhelmingly animal and in-vitro — there is no established human dose, no FDA approval, and no clinical standard for a combined blend. Everything below is concentration arithmetic, not medical advice.
How this is calculated
A blend vial behaves like any single peptide vial, except the "peptide content" is the sum of both peptides:
1. Blend concentration (mg/mL) = Total blend mg ÷ BAC water added (mL)
2. Draw volume (mL) = Your dose (mg) ÷ Blend concentration (mg/mL)
3. Units on a U-100 syringe = Draw volume (mL) × 100
The subtlety: when you draw a "1 mg blend dose" from a 5 mg / 5 mg vial, you are taking 0.5 mg BPC-157 and 0.5 mg TB-500 in that single draw — the ratio rides along automatically. If the label is 5 mg / 6 mg (11 mg total), the same 1 mg draw delivers roughly 0.45 mg BPC-157 and 0.55 mg TB-500. The syringe units only ever track the total concentration.
Blend mg → mg/mL → units
This chart reconstitutes whole blend vials with 2 mL of bacteriostatic water and shows the units for a 1 mg total-blend dose. Halve the dose, halve the units.
| Blend vial (total mg) | BAC water | Concentration | 1 mg dose draw | Units (U-100) |
|---|---|---|---|---|
| 5 mg (2.5/2.5) | 2 mL | 2.5 mg/mL | 0.40 mL | 40 units |
| 10 mg (5/5) | 2 mL | 5 mg/mL | 0.20 mL | 20 units |
| 11 mg (5/6) | 2 mL | 5.5 mg/mL | 0.18 mL | 18 units |
| 15 mg (7.5/7.5) | 2 mL | 7.5 mg/mL | 0.13 mL | 13 units |
| 20 mg (10/10) | 2 mL | 10 mg/mL | 0.10 mL | 10 units |
Worked examples
10 mg ÷ 2 mL = 5 mg/mL. Draw = 1 ÷ 5 = 0.20 mL.
Concentration still 5 mg/mL. Draw = 0.5 ÷ 5 = 0.10 mL.
20 mg ÷ 3 mL = 6.67 mg/mL. Draw = 1 ÷ 6.67 = 0.15 mL.
11 mg ÷ 2 mL = 5.5 mg/mL. Draw = 1 ÷ 5.5 = 0.182 mL.
That 1 mg draw is ~0.45 mg BPC-157 + ~0.55 mg TB-500 (the 5:6 ratio).
5 mg ÷ 1 mL = 5 mg/mL. Draw = 0.5 ÷ 5 = 0.10 mL.
15 mg ÷ 2 mL = 7.5 mg/mL. Draw = 1.5 ÷ 7.5 = 0.20 mL.
10 mg ÷ 5 mL = 2 mg/mL. Draw = 2 ÷ 2 = 1.00 mL — exactly fills a 1 mL insulin syringe.
Reconstituting the blend vial
- Read the total mg and ratio off the label (e.g. "10 mg · 5/5"). The total mg is what your maths uses.
- Wipe the septum with an alcohol swab; let it dry 10–15 seconds.
- Draw your chosen BAC water volume and inject it slowly down the inside wall of the vial. Swirl gently — never shake — until fully dissolved.
- The solution should be clear and colorless. Discard if cloudy or if particles persist.
- Label the vial with the concentration and reconstitution date, then refrigerate.
Less water means a higher mg/mL and fewer units per dose; more water means a lower mg/mL and more units. Choosing the water volume so a typical dose lands between 10 and 40 units keeps the draw easy to read.
Enter your blend's total mg, BAC water, and dose to get the exact draw in mL and units instantly.
Open the Blend Calculator →So, how do you dose a BPC-157 + TB-500 blend vial?
You divide the total blend mg by the volume of bacteriostatic water you add to get mg/mL, then divide your dose by that concentration and multiply by 100 to read the units on a U-100 insulin syringe. For a common 10 mg (5/5) vial reconstituted with 2 mL, a 1 mg dose is 5 mg/mL concentration, 0.20 mL, and 20 units. Use the BPC-157 + TB-500 blend calculator to enter your exact vial, water volume, and dose and get the draw instantly.
Frequently asked questions
How do you dose a BPC-157 + TB-500 blend vial?
Do I add the two peptides together when calculating concentration?
How is a blend vial different from using two separate vials?
What ratio is in a typical BPC-157 + TB-500 blend?
What if a dose makes the draw exceed 1 mL?
Sources
BPC-157 and TB-500 are investigational peptides; the evidence below is predominantly preclinical (animal and in-vitro). Neither is FDA-approved, and no human dose for a combined blend is established. The arithmetic here is standard concentration maths, not a clinical protocol.
- Gwyer D, Wragg NM, Wilson SL. Gastric pentadecapeptide body protection compound BPC 157 and its role in accelerating musculoskeletal soft tissue healing. Cell Tissue Res. 2019;377(2):153–159. — Review noting efficacy "yet to be confirmed in humans."
- Chang CH, Tsai WC, Lin MS, Hsu YH, Pang JH. The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration. J Appl Physiol. 2011;110(3):774–780.
- Sikiric P, Seiwerth S, Rucman R, et al. Stable gastric pentadecapeptide BPC 157: novel therapy in gastrointestinal tract. Curr Pharm Des. 2011;17(16):1612–1632.
- Goldstein AL, Hannappel E, Sosne G, Kleinman HK. Thymosin β4: a multi-functional regenerative peptide. Basic properties and clinical applications. Expert Opin Biol Ther. 2012;12(1):37–51.
- Goldstein AL, Hannappel E, Kleinman HK. Thymosin β4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421–429.
This guide is a mathematical reference only and does not constitute medical advice. BPC-157 and TB-500 are not approved for human use; consult a qualified clinician before using any injectable.